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Contract Studies

Stinging sensation
in vivo Stinging test (clinical testing)
　　n=10　no. of samples　 Primary skin irritation
Patch test with sensitive skin subjects (clinical testing)
　　n=20　no. of samples　
　　n=30　no. of samples　

Patch test (clinical testing)
　　n=20　no. of samples　
　　n=30　no. of samples　

3D Human Epidermis Model (in vitro, alternative test)
　　no. of samples　 Eye irritation（in vitro, alternative test）
STE test with monolayer SIRC cells
　　no. of samples　

RhCE test with 3D cultured cells model
　　no. of samples　 Phototoxicity (in vitro, alternative test)
ROS assay
　　no. of samples　 Single dose toxicity (in vitro, alternative test)
Cytotoxicity test using SIRC cells
　　no. of samples　
Clinical testing
anti-wrinke test (n=20, 4wk use)〈Japanese claim guideline〉
no. of samples　 Immediate effect -2hr after application
5 subjects　 
10 subjects　

Immediate effect -6hr after application
5 subjects　 
10 subjects　

Whitening and skin-improvement efficacy test (4wk use, n=10)
no. of samples　 Biochemical and Cellular Assays
Test set for 3 cosmetic domains〈whitening, anti-aging, antioxidant〉
no. of samples　

Anti-aging tests set (Collagen & hyaluronan production, fibroblast activation)
no. of samples　

Antioxidative tests set（O₂- /•OH /LOOH）
no. of samples　 Gene expression assay
8 genes/ skin whitening
no. of samples　

6 genes/anti-wrinkle, anti-aging
no. of samples　

6 genes/moisturizing and barrier functions
no. of samples　
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